Coding

Part:BBa_K1692023

Designed by: Thai Nguyen   Group: iGEM15_Stanford-Brown   (2015-09-17)
Revision as of 06:18, 22 September 2015 by ThaiN (Talk | contribs)

Ptet + Luxl

Overview

Our goal was to create an autolysis sytem that can lyse cell after cell growth reaches a threshold density. To do so, we incorporated two previous BioBricks ptet (BBa_R0040) and luxl generator (BBa_K805016). We wanted luxl to be produced constantly; however, it should not be overexpressed so we use a moderate strength promoter, ptet. We sequenced the construct and showed that it matched the intended construct. Using ptet-luxl generator, we were successful in designing our autolysis system (BBa_K1692024).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 717
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None