Part:BBa_K1694027

Pcons+B0034+FadL-GBP

Introduction

The transmembrane protein, FadL, fused with the GBP, is capable of being an anchoring motif to display GBP on the surface of E. coli. By expressing GBP out of the E. coli, GBP can recognize the surface of gold chip and specifically bind on it. If the whole chip was combined with the detector and readout parts, the antigen-antibody interaction could be detected when the gold chip acted as the transducer to transfer the trigger, mostly light, into the electric signal for nano-scale gravimetric data analysis. As the result, we may probably quantify the amounts of antigens and obtain more precise consequence.

Experiment

Fig.3 The PCR result of the Pcons+B0034+FadL-GBP. The DNA sequence length is around 1300~1500 bp, so the PCR products should appear at 1500~1700 bp.

After assemble the DNA sequences from the basic parts, we recombined each P_cons+B0034+FadL-GBP gene to PSB1C3 backbones and conducted a PCR experiment to check the size of each of the parts. The DNA sequence length of these parts is around 1300~1500 bp. In this PCR experiment, the PCR products size should be near at 1500~1700 bp. The Fig.3 showed the correct size of this part, and proved that we successful ligated the sequence onto an ideal backbone.

Fig.4 P_cons+RBS+FadL-GBP

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 499
  Illegal BamHI site found at 985
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 275
  Illegal NgoMIV site found at 767
  Illegal NgoMIV site found at 1192
  Illegal AgeI site found at 908
  
• 1000
  COMPATIBLE WITH RFC[1000]