Coding

Part:BBa_K1639016:Design

Designed by: Berke Kahraman   Group: iGEM15_ATOMS-Turkiye   (2015-09-16)
Revision as of 23:29, 21 September 2015 by Nurish (Talk | contribs) (→‎Source)

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VP16 Transcriptional Activation Domain with miR223 and miR21 Binding Site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 349
    Illegal PstI site found at 355
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 349
    Illegal PstI site found at 355
    Illegal NotI site found at 320
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 349
    Illegal PstI site found at 355
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 349
    Illegal PstI site found at 355
    Illegal NgoMIV site found at 783
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 313


Design Notes

This part was designed without prefix and suffix. It's composed of small portion of tTA from pTet-off plasmid followed up by miR binding sites and SV40 polyA signal. We used this gene to express tTA protein but with miR binding sites. 1.Clone into pTet-off with Sal1 and Hind3 enzymes 2.Clone into pSB1C3 with appropiate enzymes located on vector

Source

It's a modified version of pTet-Off plasmid

References