Part:BBa_K1615045

Heroin Esterase in RFC25

Heroin esterase, an acetylmorphine carboxylesterase, was isolated from Rhodococcus erythropolis strain H1 in 1994 from the garden soil at Cambridge and is able to use heroin as its sole carbon and energy source by deacetylating the C-3 and C-6 groups to form morphine¹. The gene her encodes this enzyme and can be expressed in the chassis Escherichia coli². The pH optimum for this enzyme is pH8.5 in bicine buffer¹.



The activity of heroin esterase can be tested using 4-nitrophenyl acetate which is hydrolysed by heroin esterase to form 4-nitrophenol and acetate³. This produces a yellow colour which can be read at 410 nm.

The following table summarises the kinetic analysis and statistics of the measurement for heroin esterase.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 21
  Illegal BamHI site found at 777
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]