Coding

Part:BBa_K1639008:Design

Designed by: Mustafa Yılmaz   Group: iGEM15_ATOMS-Turkiye   (2015-09-13)
Revision as of 13:20, 21 September 2015 by Mustafa26 (Talk | contribs) (→‎Design Notes)

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Tev Protease, single S219V mutation in the internal cleavage site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2
    Illegal XhoI site found at 744
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 605


Design Notes

This part contains an additional BamHI restriction site at beginning of Tev Protease sequence and a XhoI restriction site at the end.

Source

Tobacco etch virus (TEV).

References