Composite

Part:BBa_K1618020

Designed by: Kieran Rustage   Group: iGEM15_NRP-UEA-Norwich   (2015-08-28)
Revision as of 13:00, 21 September 2015 by Nicolapatron (Talk | contribs)

Plac-Buk-PTB

K1618020 contains the coding sequences for butyrate kinase and phosphotransbutrylase from Coprococcus sp. (L2-50 DSM 16842) under control of the LacI promoter. This part should catalyze the conversion of Butyrate to/from Butyryl-CoA though an intermediate of butyryl.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 508
    Illegal BglII site found at 748
    Illegal BglII site found at 889
    Illegal BglII site found at 1623
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1449


[edit]
Categories
Parameters
None