Composite
CYP11A1

Part:BBa_K1598008:Design

Designed by: Yash Mishra   Group: iGEM15_UCL   (2015-09-18)
Revision as of 09:55, 21 September 2015 by Yashmishra (Talk | contribs) (→‎Source)


J23101 promoter + RBS + Human FDXR + RBS + Human FDX1 + RBS + Human CYP11A1 + rrnb double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3275
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 565
    Illegal XhoI site found at 1457
    Illegal XhoI site found at 3710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1653
    Illegal AgeI site found at 1090
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to make sure that that the genes for Cytochrome P450scc and all it's electron transport proteins were included in the biobrick and came after ribosome binding sites. The biobrick was broken into 5 parts for quick synthesize and assembled using Golden Gate assembly. Hence, each part was made compatible for golden gate assembly, and had overhangs for SapI.


Source

http://www.ncbi.nlm.nih.gov/nuccore/153218645?report=fasta

http://www.ncbi.nlm.nih.gov/nuccore/16877631?report=fasta

http://www.ncbi.nlm.nih.gov/nuccore/39645178?report=fasta

https://parts.igem.org/Part:BBa_J34801

https://parts.igem.org/Part:BBa_J23101

http://file.scirp.org/Html/38129.html

References