Part:BBa_K1846001:Design

Bacteriophage lambda tail fibre assembly protein circuit

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This tfa circuit was synthesised removing the illegal restriction sites to make the sequence BioBrick-compatible. The sequence was cloned into a pSB1C3 and the success of the cloning procedure was confirmed by restriction with EcoRI and PstI restriction enzymes and followed by agarose gel electrophoresis (Figure 1). The construct was also confirmed by sequencing. This BioBrick was registered as part BBa_K1846001.

Source

DNA synthesis