Part:BBa_K549001:Experience

Our first experiment with this BioBrick showed positive results. Hypothetically the linear response for this system is fulfilled for a concentration range from 0.5 to 60 µM. But only to see, whether the BioBrick works or not, we started a pretest to see a yes-or-no-answer. For this we induced the system with a nickel-concentration lying high above the said concentration range and measured the output when the colony was in its stationary phase. As you can clearly see here in figure 1b), nearly all of the cells gave an apparent output.

Figure 1: with BBa_K549001 transformed stationary phase E.coli cells, cultivated in LB-media (containing Ni(II)) a) left hand side: phase contrast microscopy b)right-hand side: fluorescence microscopy GFP-excitation

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Team Bielefeld-CeBiTec 2015

We used a modified version of team LMU Munich 2011´s nickel biosensor. We used an E. coli codon optimzed version of the repressor BBa_K1758350 and combined it with their lead responsive promoter which is upstrem of a expression enhancing 5´UTR and sfGFP BBa_K1758352. Using this two we tried to creat a device for nickel detection BBa_K1758353. Our data suggest that this does not work as expected.