DNA

Part:BBa_K1614022

Designed by: Daniel Heid   Group: iGEM15_Heidelberg   (2015-09-18)
Revision as of 19:36, 20 September 2015 by MAJendrusch (Talk | contribs)

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p53 AptaBody

MAWS software generated aptamer for the tetramerization domain of p53 linked with a 5x A-Linker to the HRP-mimicking DNAzyme.

This part comprises an aptamer designed to targed p53 using MAWS a linker of AAAAA and the HRP-DNAzyme. It is capable of providing luminescence readout in the presence of hydrogen peroxide, and designed for Western blotting. Degradation of hydrogen peroxide needed for luminescent readout with luminol requires the presence of hemin, with optimal behaviour at equimolar concentration of hemin and the construct. The optimal pH for the HRP-DNAzyme used in this construct is pH 8.5.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None