Coding

Part:BBa_K773002

Designed by: Katie Knister   Group: iGEM12_Caltech   (2012-10-01)
Revision as of 15:25, 20 September 2015 by Cridelbianco (Talk | contribs) (→‎Usage and Biology)

Proteorhodopsin

This part carries the gene for a proteorhodopsin pump, which can generate a proton motive force for making ATP.


Usage and Biology

This part was improved by Silvia Galvan of the iGEM UniTN Trento 2015 team. This part was previously found by other users to not express protein nor produce ATP. We improved this part by adding a strong RBS and using a different inducible promoter (arabinose, araC-pBAD). We were able to express the protein and functionally characterize it. The part now works! Check the experience page of this part to see our data.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 515
    Illegal XhoI site found at 88
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 277
    Illegal AgeI site found at 652
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None