RNA

Part:BBa_K1614018

Designed by: Daniel Heid   Group: iGEM15_Heidelberg   (2015-09-18)
Revision as of 14:17, 20 September 2015 by Frieda (Talk | contribs)

ATP Aptamer Spinach 2.1

The ATP Aptamer Spinach2.1 is the first fluorescent ATP sensor that was designed for the in vitro transcription tool box designed by the iGEM Team Heidelberg 2015. This ATP Aptamer Spinach2.1 is cloned into the RFC 110.

Usage and Biology

ATP Aptamer Spinach 2.1 is a fusion of the BBa_K1330000 generated by DTU Danemark. According to our results, this fusion should contain the same properties as BBa_K1614012. Yet, this Spinach 2.1 is ligand dependend to ATP and should find its use in the detection of the small molecule ATP. Applications such as nucleotide sensing during in vitro transcription are possible. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 39
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None