Reporter
Part:BBa_I13602
Designed by: Christopher Batten, Victoria Chou, Kenneth Nesmith Group: Antiquity (2004-07-16)
Tet operator with CFP reporter (with LVA tag) [R/Tc+]
Usage and Biology
This part can be used to test the classical Tet repression system with a CFP reporter. The Ptet promoter used is a medium strength promoter.
Figure 1: Fluorescence microscopy of E. coli DH5alpha bearing I13602
(A) Differential interference contrast (B) excitation with led 437 nm/17nm (CFP), 200 ms of exposure
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//classic/reporter/pret
emission | cyan |
excitation | |
tag | LVA |