Reporter

Part:BBa_I13602

Designed by: Christopher Batten, Victoria Chou, Kenneth Nesmith   Group: Antiquity   (2004-07-16)
Revision as of 12:59, 20 September 2015 by AudreyMo (Talk | contribs) (→‎Usage and Biology)

Tet operator with CFP reporter (with LVA tag) [R/Tc+]



Usage and Biology

This part can be used to test the classical Tet repression system with a CFP reporter. The Ptet promoter used is a medium strength promoter.

Figure 1: Fluorescence microscopy of E. coli DH5alpha bearing I13602

Paris Saclay-I13602.png

A) Differential interference contrast

B) excitation with led 437 nm/17nm (CFP), 200 ms of exposure


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/reporter/pret
Parameters
emissioncyan
excitation
tagLVA