Device
Ouroboros

Part:BBa_K1777016:Design

Designed by: Xilin Jiang   Group: iGEM15_Fudan   (2015-09-17)
Revision as of 11:51, 20 September 2015 by WooYehphan (Talk | contribs) (→‎Source)


Device to produce circRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 462
    Illegal XhoI site found at 833
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 804


Design Notes

This device is specially designed based on back-splicing mechanism.


Source

We build this two device with BBa_K1777015 and BBa_K1777003, as well as beta-globin intron(BBa_K404107). We used the β-globin intron to increase the expression level of mRNA and provide SA and SD site to help the cirlization of mRNA at the same time.

References