Part:BBa_K1694037

Pcons+B0034+FadL-GBP+B0030+GFP+J61048

Introduction

The transmembrane protein, FadL, fused with the GBP, can act as an anchoring motif for displaying GBP on the surface of E.coli. To achieve the convenient observation, the genetic sequence of green fluorescent protein was introduced in our design. With the dual display of the GBP and the GFP, the E.coli can not only bind on the gold surface but also produce green fluorescent signal.

Experiment

We intended to make the observation of the result more directly by producing green fluorescence at the same time. To make the comparison of the binding efficiency, we selected the genetic sequence, Pcons + rbs +GFP +ter, as the negative control group. Because there was no existence of fully-functioned phenomenon, there was no positive control in our experiment.

Fig.5(a)the results of the test group, which contains the genetic sequence of Pcon+ rbs + FadL-3rdGBP + rbs + GFP + ter

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 499
  Illegal BamHI site found at 985
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 275
  Illegal NgoMIV site found at 767
  Illegal NgoMIV site found at 1192
  Illegal NgoMIV site found at 2102
  Illegal AgeI site found at 908
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2018