Coding
tetA(C)b
Part:BBa_J31006
Designed by: Sabriya Rosemond, Erin Zwack Group: iGEM06_Davidson (2006-07-12)
tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]
This is the coding region for tetracyline resistance [TetA(C) inner-membrane-associated protein] in the reverse orientation. This does not code for the repressor of the tetracycline promoter. Please read the note about this part's BioBrick cloning sites on the part design page.
Usage and Biology
If RBS (reverse) and a promoter (reverse) are placed to the right of this part, the cell will express tetracycline resistance.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1043
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 897
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 343
Illegal NgoMIV site found at 503
Illegal NgoMIV site found at 871 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/selectionmarker/antibioticresistance
function | Rep |
ligands | tetracycline |
protein | TetA(C)-bkw |
swisspro | P02981 |