Composite

Part:BBa_K1694055

Designed by: CHIH-HSUAN HSU   Group: iGEM15_NCTU_Formosa   (2015-09-15)
Revision as of 10:02, 20 September 2015 by LukeChang (Talk | contribs)

Pcons+B0034+Lpp-OmpA-N+scFv(Anti-HER2)+B0034+amilCP+B0015

Introduction

To observe that our scFv can bind onto the cancer cells, we connected each scFv with different chromoprotein. Color proteins are commonly used as reporter gene for observation. However, chromoproteins can be conveniently observed by naked eye without the help of instruments. Therefore, when we conducted cell staining experiment, we can observe the binding distribution by color right after the experiments have finished.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 741
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 451
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None