Composite
Part:BBa_K1675008
Designed by: JIAQI XU Group: iGEM15_BIT-China (2015-09-15)
A device used in the synthesis of lactic acid
L-Lactic acid, one of the most important chiral molecules and organic acids, is produced via pyruvate from carbohydrates in diverse microorganisms catalyzed by an NAD-dependent L-lactate dehydrogenase. The LdhA gene is used in the synthesis of lactic acid. This gene encodes an L-lactate dehydrogenase (L-LDH) which converts pyruvate to L-lactate.
To verified the LdhA gene, we constructed our gene into pET28a (Fig.1), and induced its over-expression by IPTG (Isopropyl β-D-1-Thiogalactopyranoside) under 16 ℃ in BL21(DE)3.
Fig.1 The constructed result of LdhA-pET28a
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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