Composite

Part:BBa_K1692021:Design

Designed by: Erica Lieberman   Group: iGEM15_Stanford-Brown   (2015-08-03)
Revision as of 09:34, 20 September 2015 by EricaKL (Talk | contribs) (→‎Source)

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PanK + hybrid promoter phaCAB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
    Illegal NheI site found at 912
    Illegal NheI site found at 935
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1884
    Illegal BglII site found at 2709
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1190
    Illegal NgoMIV site found at 1261
    Illegal NgoMIV site found at 1861
    Illegal NgoMIV site found at 2173
    Illegal NgoMIV site found at 2452
    Illegal NgoMIV site found at 3104
    Illegal NgoMIV site found at 3126
    Illegal AgeI site found at 418
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4970


Design Notes

Since the gene is from S. aureus, we codon-optimized it for production in E. coli.

Thank you to our advisor, Dr. Kosuke Fujishima for assistance in designing this BioBrick.

Source

[http://www.uniprot.org/uniprot/Q2FWC7 Uniprot] and Imperial College iGEM team 2013

References