Part:BBa_K1675008

A device used in the synthesis of lactic acid

L-Lactic acid, one of the most important chiral molecules and organic acids, is produced via pyruvate from carbohydrates in diverse microorganisms catalyzed by an NAD-dependent L-lactate dehydrogenase. The LdhA gene is used in the synthesis of lactic acid. This gene encodes an L-lactate dehydrogenase (L-LDH) which converts pyruvate to L-lactate.

To verified the LdhA gene, we constructed our gene into pET28a (Fig.1,2), and induced its over-expression by IPTG (Isopropyl β-D-1-Thiogalactopyranoside) under 16 ℃ in BL21(DE)3.

Fig.2 The plasmid pET28a and gene LdhA

The constructed result of LdhA-pET28a

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]