Composite

Part:BBa_K1675007

Designed by: Hui Luo   Group: iGEM15_BIT-China   (2015-09-11)
Revision as of 09:23, 20 September 2015 by JIAQIXU (Talk | contribs)

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a device to express the glutaminase1 constitutively

Uses a constitutive promoter(BBa_J23119), strong RBS(BBa_B0034),glsA coding region(BBa_K1675002).Will generate glutaminase constitutively.

After the construction of acid-resistance circuit, we measured the OD600 under different pH to test the efficacy of acid resistance. The growth curve is shown in Fig.1.

BIT China Resistance System pic11.jpg

Fig.1 The growth curve of experimental group(JBG) and control


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
biology