Coding

Part:BBa_K1777019:Design

Designed by: Xuhang Li   Group: iGEM15_Fudan   (2015-09-18)
Revision as of 08:12, 20 September 2015 by Xuhangli (Talk | contribs) (→‎References)

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flexible linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we chose a linker for our fusion protein，we would have wanted to use -gggsgggs- linker(BBa_K1486003). But we found that such a tandem repeating linker is hard to amplify via PCR because the primer binding site should be unique. Therefore, we improved the original part by change the first GGGS to a carefully selected natural linker from linker database[1] to avoid repeating sequence. Besides, we used the selected natural linker to adjust its length to about 50 Angstrom in total.

Source

BBa_K1486003

References

1.linker database(http://www.ibi.vu.nl/programs/linkerdbwww/） George RA. and Heringa J. (2002) An analysis of protein domain linkers: their classification and role in protein folding. Protein Engineering 15, 871-879
2.link BBa_K1486003