Protein_Domain

Part:BBa_K1486003

Designed by: iGem EPFL 2014   Group: iGEM14_EPF_Lausanne   (2014-08-12)
Revision as of 08:02, 20 September 2015 by Jxl (Talk | contribs)

Flexible linker 2x (GGGS)

Flexible linker used to fuse two proteins.
Group: iGEM15_Fudan
Author: Xuhang Li/n
Summary:We developed a new part(BBa_K1777005) based on this parts. The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//direction/forward
//proteindomain/linker
Parameters
chassisE coli
device_typelinker
directionforward