DNA

Part:BBa_K1648005:Experience

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-09-15)
Revision as of 05:31, 20 September 2015 by Fatfaatlai (Talk | contribs)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1648005

The GFP-nanobody is PCR from Anti-GFP Nanobody with IgG kappa signal peptide and IgG1 Fc domain [K929102]. PCR GFPnanobody.jpg

Figure 1. The photo of 1% agarose gel electrophoresis showing PCR products.

L: DNA ladder. Lane 2: GFP-nanobody.


This Part is added to Insertion Kit(K1648004), forming a new Part(K1648006), which is used to express GFP-nanobody on the membrane of magnetosome.

This Part were verified by double digestion (Fig. 2) and sequencing.

GFPnano-c.JPG

Figure 2. Checking of recombinant plasmid using double digestion. L: DNA ladder. Lane 1-3: GFP- nanobody (BBa_K1648005) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site.

User Reviews

UNIQee8331a546e25d8c-partinfo-00000000-QINU UNIQee8331a546e25d8c-partinfo-00000001-QINU