Composite
J13002AvOp

Part:BBa_K1648048:Experience

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-05-31)
Revision as of 05:02, 20 September 2015 by Alyssaliu (Talk | contribs)

Double digestion of K1648048: L: DNA ladder. Lane 1-3: Recombination Template for J13002-oprF(BBa_K1648048) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site, without digestion.


















Applications of BBa_K1648048

An ongoing experiment will be carried out for the comparison between the transformed bacteria with BBa_K1648048 for oprF from Azotobacter vinelandii BBa_K1648049 for mutated oprF from Azotobacter vinelandii and BBa_K1648050 for BBa_K1172501 from Germany iGEM team. For comparison,an identical promoter, J13002, which is a constitutive promoter, is chosen to add before the gene in psb1C3 for constitutive expression of different OprF in bacteria.

User Reviews

UNIQb7bc0482e83c2e14-partinfo-00000000-QINU UNIQb7bc0482e83c2e14-partinfo-00000001-QINU