Reporter
Part:BBa_K1676100
Designed by: Ooi Kean Hean Group: iGEM15_NTU-Singapore (2015-09-18)
GFP Reporter with pLac as promoter
For GFP measurement as control. Our team, NTU igem has created a single basepair mutant library for the RBS, BBa_B0034. We have characterised this mutant in Shewanella oneidensis MR1.
Mutant library constructed via site directed mutagenesis:
Shown below are the mutants of the RBS with the same reporter. 1AT denote substitution of A to T.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 725
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Categories
Parameters
None |