Coding

Part:BBa_K1692020

Designed by: Erica Lieberman   Group: iGEM15_Stanford-Brown   (2015-08-03)
Revision as of 03:36, 20 September 2015 by EricaKL (Talk | contribs)

S. aureus type II PanK (CoaA)

This basic part is type II pantothenate kinase (panK) from Staphylococcus aureus, which means that it is not sensitive to feedback inhibition from coenzyme A or acetyl-CoA. This means that high levels of coenzyme A accumulate. We did not experiment with this gene alone; instead, we incorporated it into an already-existing biobrick (BBa_K1149051) to increase the production of poly-3-hydroxybuterate, a plastic of which coenzyme A is a precursor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 10
    Illegal NheI site found at 33
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 420
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None