Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Composite

Part:BBa_K1806005

Designed by: İBRAHİM YASİR ORHAN   Group: iGEM15_AUC_TURKEY   (2015-09-17)
Revision as of 00:38, 20 September 2015 by TLOS (Talk | contribs)

T7+ ibPB RNA Thermometer + RFP

The part is designed to produce RFP in higher temperatures. The RNA Thermometer acts as a heat-shock RBS.

Cloning

The already linear part was ligated to the pSB1C3 vector, and then transformated to be verified with Colony PCR. The RFP in the iGEM Registry contained Hind3, unlike the RFP in this part. For this reason, the part was cut with the EcoR1 and Hind3 restriction enzymes. The gel runs of the part were examined. Sample 7-6 yielded the accurate base pair length.

6005-1.png 6005-2.png

6005-3.png 6005-4.png

Functional Assay

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 720
    Illegal AgeI site found at 832
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/composite_part/nominee
Parameters
n/aT7+ ibPB RNA Thermometer + RFP