Reporter

Part:BBa_K1676057

Designed by: Ooi Kean Hean   Group: iGEM15_NTU-Singapore   (2015-09-16)
Revision as of 16:29, 19 September 2015 by Yinyuan1 1 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

GFP Reporter with Mutant RBS 7GT

This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the seventh base pair. The base pair G is substituted to T.

The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , GFP BBa_E0040 and double terminator BBa_B0015.

The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.

This reporter can also be derived from the existing construct, BBa_J69511.

The characterization is shown below,

Growth curve:

789gc.png

GFP Reading:

Gfp789.png

It is shown to have a fluorescence reading lower than the wild type RBS reporter, BBa_J69511.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725


[edit]
Categories
Parameters
None