Composite
cI-Cro+

Part:BBa_K1846005

Designed by: Luba Prout, Vitor Pinheiro   Group: iGEM15_Birkbeck   (2015-09-17)
Revision as of 12:57, 19 September 2015 by LubaProut (Talk | contribs)

cI-Cro bacteriophage lambda lytic cycle regulatory circuit

The cI-Cro construct contains a circuit for the production - via a constitutive promoter - of the cI repressor protein (also known as the Lambda Repressor), which is responsible for keeping bacteriophage lambda in the lysogenic cycle through the cooperative binding of two repressor dimers to the DNA, repressing the Cro gene. The circuit uses a T7 promoter to drive the expression of the Cro gene in the opposite direction to the cI gene, essentially silensing the expression of the cI gene. Thus, the presence of the T7 RNA polymerase in the system enables the switch from the lysogenic cycle to the lytic cycle.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None