DNA

Part:BBa_K1648005

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-09-15)
Revision as of 12:28, 19 September 2015 by Fatfaatlai (Talk | contribs)

GFP-nanobody

The GFP-nanobody is PCR from Anti-GFP Nanobody with IgG kappa signal peptide and IgG1 Fc domain [K929102]. PCR GFPnanobody.jpg

Figure 1. The photo of 1% agarose gel electrophoresis showing PCR products.

L: DNA ladder. Lane 2: GFP-nanobody.


This Part were verified by double digestion (Fig. 2) and sequencing.

GFPnano-c.JPG

Figure 2. Checking of recombinant plasmid using double digestion. L: DNA ladder. Lane 1-3: GFP- nanobody (BBa_K1648005) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aGFP-nanobody