Reporter

Part:BBa_K1676037

Designed by: Ooi Kean Hean   Group: iGEM15_NTU-Singapore   (2015-09-09)
Revision as of 09:43, 19 September 2015 by OOIK0003 (Talk | contribs)

GFP Reporter with Mutant RBS 1AC

This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the second base pair. The second base pair A is substituted to C. The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , eGFP BBa_E0040 and double terminator BBa_B0015.

The purpose of this construct is to characterise the mutant RBS in Shewanella Oneidensis MR1 strain.

This reporter can also be derived from the existing construct, BBa_J69511.

The characterisation is shown below,

Growth curve:

123_gc.png

GFP Reading:

123.png

It is shown to have a fluorescence reading higher than the wild type RBS reporter, BBa_J69511.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725


[edit]
Categories
Parameters
None