Generator

Part:BBa_K1758323

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-08-29)
Revision as of 05:53, 19 September 2015 by Gila (Talk | contribs) (→‎Usage and Biology)

UTR-sfGFP under control of Copper responsive promoter

Copper induceble promoter with an untranslated region and sfGFP for detection via fluorescence

Usage and Biology

CopAP is the central component in obtaining copper homeostasis, which exports free copper from cytoplasm to the periplasm. This is enabled by copper induced activation of the operon transcription via CueR. The CueR-Cu+ is the DNA-binding transcriptional dual regulator which activates transcription (Yamamoto, Ishihama 2005). In our project this part is essential for the in vitro characterization of our copper sensor. We started characterizing it with this device, but data suggested that we could reach higher fluorescence level using a T7 promoter, which was realized in BBa_K1758325.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 29
    Illegal SapI.rc site found at 179


[edit]
Categories
//function/sensor/metal
Parameters
None