Generator

Part:BBa_K1631019

Designed by: Yuto Yamanaka   Group: iGEM15_UT-Tokyo   (2015-09-14)
Revision as of 03:30, 19 September 2015 by Yamanaka (Talk | contribs) (→‎Usage and Biology)

Pconst(medium) -rbs- cheZ -d.term

Constitutive CheZ generator (medium).


Usage and Biology

We constructed new composite CheZ generators(CheZ strong1 generator Part:BBa_K1631018, CheZ strong2 generator part:BBa_K1631019, Medium CheZ generatorpart:BBa_K1631020) and introduced them to CheZ knockout strain named JW1870 (Keio collection). In contrast to previous iGEM projects, we tried to measure diffusion rate of E.coli quantitavely. In this end we took time lapse photography to measure the colony expanding speed. By combination with measurement of growth rate we could successfully quantitate diffusion rates according to different strength of CheZ expression.

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/motility
Parameters
None