Inverter

Part:BBa_J5516

Designed by: Charles Yoon   Group: iGEM06_Toronto   (2006-10-29)
Revision as of 20:55, 9 April 2007 by Drew (Talk | contribs)

Arabinose -> LacI ts -| LacI+pL

WARNING: This is not a PoPS-based inverter!

Increasing concentration of Arabinose should increase LacI ts output. As LacI ts forms a tetramer, it will repress the pLac promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2383
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None