Part:BBa_K1602018

Humicola insolens cutinase with N-terminal His-Tag

The Humicola insolens cutinase (HIC) (EC 3.1.1.74, cutinase) belongs to the serine-α -β-hydrolase family (Nielsen et al., 2005) and can be used to degrade polymers like polyethylene terephthalate (Espino-Rammer et al., 2013). The catalytic pocket consists of the triad of a serine, histidine and aspartic acid (Kold et al., 2014). In addition it was reported that HIC catalyze condensation and lactone ring-opening polymerizations in an organic phase (Hunsen et al., 2008) at an optimal activity of 70°C. Assays showed no ester cleavage activity after incubating at 70°C for 15 min. An N-terminal attached Polyhistag enables purification via immobilized metal affinity chromatography (IMAC).

Characteristics

Molecular Weight	21068.85
Residues	200
Charge	5.5
Isoelectric Point	8.1624
A280 Molar Extinction Coefficients	12950 (reduced) 13200 (cystine bridges)
Improbability of expression in inclusion bodies	0.891
[Data taken from PEPSTATS]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]