Coding

Part:BBa_K1760001:Design

Designed by: Anthony Zhao, Seray Cicek, Umar Owadally, Katariina Jaenes, Matt D'lorio, ChungMin Lee, Drupad Rajyaguru   Group: iGEM15_Toronto   (2015-09-18)
Revision as of 02:36, 19 September 2015 by Cicekser (Talk | contribs) (→‎Design Notes)

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TodE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 997
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This design contains: a constitutive TetO promoter without TetR gene, an RBS designed by using Salis RBS calculator and a coding todE sequence codon optimized for E.Coli

This design is Gibson Assembled.

Source

Pseudomonas putida F1 (Synthesized)

References