Regulatory

Part:BBa_K1728002

Designed by: Yung-Li Chen, Timothy Chan En Haw   Group: iGEM15_CGU_Taiwan   (2015-09-16)
Revision as of 02:35, 19 September 2015 by Allen (Talk | contribs) (→‎Usage and Biology)

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DUSP1 toehold switch RNA sensor

DUSP1 toehold switch RNA sensor


Usage and Biology

Toehold switch is a secondary structure of RNA. Because of special sequence design, it can form a hairpin structure and hide ribosome binding site (RBS)in the loop. There are three basic elements of toehold switches including 30bp trigger sequence(AAATAAATTGAAGTGGGCTCAAGGAGACCC), bacteria ribosome binding site and linker.The trigger sequence of this part is complementary to a part of Dual specificity protein phosphatase 1 (DUSP1) mRNA(DUSP1 partial sequence, BBa_K1728006). Once the DUSP1 mRNA is presented, the toehold structure will open. Therefore, the ribosome is able to bind on the RBS and translate the down stream gene.


Toehold switch secondary structure prediction

CGU_DUSP1.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 24


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
None