Coding

Part:BBa_K1796006

Designed by: Nannan Xie   Group: iGEM15_SCU_China   (2015-09-15)
Revision as of 02:26, 19 September 2015 by Nieyi (Talk | contribs) (→‎Parameter of Protein)

Dinitrogenase reductase(nifH)

The nifH encoding dinitrogenase reductase(also called Fe protein) is an important gene in nitrogen fixation. The protein is a homodimer bridged by an intersubunit [4Fe-4S] cluster that serves as the obligate electron donor to the MoFe protein. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Parameter of Protein

Number of amino acids: 288

Molecular weight: 31494.9

Theoretical pI: 4.78

Amino acid composition:

Ala (A) 26 9.0%

Arg (R) 13 4.5%

Asn (N) 15 5.2%

Asp (D) 14 4.9%

Cys (C) 6 2.1%

Gln (Q) 13 4.5%

Glu (E) 28 9.7%

Gly (G) 28 9.7%

His (H) 4 1.4%

Ile (I) 22 7.6%

Leu (L) 27 9.4%

Lys (K) 14 4.9%

Met (M) 11 3.8%

Phe (F) 8 2.8%

Pro (P) 8 2.8%

Ser (S) 9 3.1%

Thr (T) 17 5.9%

Trp (W) 0 0.0%

Tyr (Y) 8 2.8%

Val (V) 17 5.9%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 42

Total number of positively charged residues (Arg + Lys): 27

Atomic composition:Carbon C 1372

Hydrogen H 2213

Nitrogen N 377

Oxygen O 435

Sulfur S 17

Formula: C1372H2213N377O435S17Total number of atoms: 4414

Extinction coefficients:This protein does not contain any Trp residues. Experience shows that

this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 12295

Abs 0.1% (=1 g/l) 0.390, assuming all pairs of Cys residues form cystines

Ext. coefficient 11920

Abs 0.1% (=1 g/l) 0.378, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 39.05

This classifies the protein as stable.

Aliphatic index: 92.50

Grand average of hydropathicity (GRAVY): -0.161

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