Translational_Unit

Part:BBa_K1679013:Design

Designed by: Cun Wei   Group: iGEM15_OUC-China   (2015-09-07)
Revision as of 02:25, 19 September 2015 by J3n (Talk | contribs) (→‎Design Notes)

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RNA thermometer (ROSE)+mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 657
    Illegal AgeI site found at 769
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 42°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above 42°C, the secondary structure of the RBS after transform would be unfolded and produce RFP. We designed this part for measurement of the switch activity of K115001.

Source

BBa_K115001 BBa_E1010

References

Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265.