Translational_Unit

Part:BBa_K1679014:Design

Designed by: Cun Wei   Group: iGEM15_OUC-China   (2015-09-07)
Revision as of 02:24, 19 September 2015 by J3n (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

RNA thermometer (FourU) +mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 613
    Illegal AgeI site found at 725
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 37°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above 37°C, the secondary structure of the RBS after transform would be unfolded and produce RFP. We designed this part for measurement of the switch activity of K115002.

Source

BBa_K115002 BBa_E1010

References

Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265.