Coding
HisTag-HIC

Part:BBa_K1602018

Designed by: Sebastian Barthel, Jonas Sindlinger, Bianca Reisinger, Sven Jager   Group: iGEM15_TU_Darmstadt   (2015-09-16)
Revision as of 02:06, 19 September 2015 by S Barthel (Talk | contribs) (→‎Characteristics)

Humicola insolens cutinase with N-terminal His-Tag

The Humicola insolens cutinase (HIC) (EC 3.1.1.74, cutinase) belongs to the serine-α -β-hydrolase family (Nielsen et al., 2005) and can be used to degrade polymers like polyethylene terephthalate (Espino-Rammer et al., 2013). The catalytic pocket consists of the triad of a serine, histidine and aspartic acid (Kold et al., 2014). In addition it was reported that HIC catalyze condensation and lactone ring-opening polymerizations in an organic phase (Hunsen et al., 2008) at an optimal activity of 70°C. Assays showed no ester cleavage activity after incubating at 70°C for 15 min.

Characteristics

Molecular Weight 21068.85
Residues 200
Charge 5.5
Isoelectric Point 8.1624
A280 Molar Extinction Coefficients 12950 (reduced) 13200 (cystine bridges)
Improbability of expression in inclusion bodies 0.891
[Data taken from PEPSTATS]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None