Plasmid

Part:BBa_K1760000:Design

Designed by: Seray Cicek, Umar Owadally, Katariina Jeanes, ChungMin Lee, Anthony Zhao   Group: iGEM15_Toronto   (2015-09-18)
Revision as of 02:06, 19 September 2015 by Cicekser (Talk | contribs) (→‎Design Notes)

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TodE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1
    Illegal EcoRI site found at 2049
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2049
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2049
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 1925
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2
    Illegal EcoRI site found at 2049
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2049
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 3055
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed biobrick is a composite part that contains a TetO promoter, RBS optimized by using Salis RBS calculator and codon optimized todE gene for E.Coli. TodE gene is part of a tod operon found in Pseudomonas Putida F1 species. It plays a major role in degradation of toluene. Specifically, todE degrades 3-methylcatechol, a highly toxic intermediate into HOD. In pseudomonas putida species, HOD is aerobically metabolized through TCA cycle. Theoretically, a E.Coli MG1655 type bacteria containing todC1C2BADEF genes can degrade toluene through TCA cycle by using its own native enzymes.

Source

Pseudomonas putida F1 (Synthesized)

References