Part:BBa_K1602018

Humicola insolens cutinase with N-terminal His-Tag

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The Humicola insolens Cutinase (HIC) (EC 3.1.1.74, cutinase) belongs to the serine-α -β-hydrolase family (Nielsen et al., 2005) and can be used to degrade polymers like polyethylene terephthalate (Espino-Rammer et al., 2013). The catalytic pocket consists of the triad of a serine, histidine and aspartic acid (Kold et al., 2014) and the enzyme has its optimal activity at 70°C (Hunsen et al., 2008). Characteristics Molecular Weight 21068.85 Residues 200 Charge 5.5 Isoelectric Point 8.1624 A280 Molar Extinction Coefficients 12950 (reduced) 13200 (cystine bridges) Improbability of expression in inclusion bodies 0.891 [Data taken from PEPSTATS] Sequence and Features Assembly Compatibility: 10 COMPATIBLE WITH RFC[10] 12 COMPATIBLE WITH RFC[12] 21 COMPATIBLE WITH RFC[21] 23 COMPATIBLE WITH RFC[23] 25 COMPATIBLE WITH RFC[25] 1000 COMPATIBLE WITH RFC[1000]