Part:BBa_K1640018
CHlM
ChlM gene from Chlamydomonas reinhardtii, encodes S-Adenosyl-L-methionine:magnesium protoporphyrin IX methyltransferase, a protein involved in chlorophyll biosynthesis.
Usage and Biology
The autotrophic gene ChlM is involved in the chlorophyll biosynthetic pathway in the formation of chlorophyll a (Meinecke et al. 2010). It encodes the enzyme magnesium protoporphyrin IX methyltransferase (MgPMT), involved in the second step of chlorophyll biosynthesis from protoporphyrin IX (Block et al, 2002). ChlM utilizes ubiquitous methylating agent S-Adenosyl-L-methionine (SAM) as a cofactor in the catalysis of Mg-protoporphyrin IX through methyl group transfer, forming Mg-protoporyphyrin IX monomethyl ester (Gibson et al., 1963).
S-adenosyl-L-methionine + magnesium protoporphyrin IX ⇌ S-adenosyl-L-homocysteine + magnesium protoporphyrin IX 13-methyl ester
This part allows E. coli to express the Chlamydomonas reinhardtii-derived ChlM gene involved in the chlorophyll biosynthesis pathway.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 225
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Block, M., Tewari, A., Albrieux, C., Maréchal, E. and Joyard, J. (2002). The plant S-adenosyl-l-methionine:Mg-protoporphyrin IX methyltransferase is located in both envelope and thylakoid chloroplast membranes. European Journal of Biochemistry, 269(1): 240-248. Gibson, K.D., Neuberger, A. and Tait, G.H. (1963). Studies on the biosynthesis of porphyrin and bacteriochlorophyll by Rhodopseudomonas spheroides. 4. S-Adenosylmethionine–magnesium protoporphyrin methyltransferase. Biochemical Journal, 88(2): 325-334.
Meinecke, L., Alawady, A., Schroda, M., Willows, R., Kobayashi, M., Niyogi, K., Grimm, B. and Beck, C. (2010). Chlorophyll-deficient mutants of Chlamydomonas reinhardtii that accumulate magnesium protoporphyrin IX. Plant molecular biology, 72(6): 643-658.
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