Coding

Part:BBa_K1813051

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-09-11)
Revision as of 01:47, 19 September 2015 by Ishd (Talk | contribs)

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CYP6G1 with pelB Expression Cassette

Coding sequence of CYP6CM1vQ ( BBa_K1813003) prefixed by the pelB localization signal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


Background

The pelB localization signal targets a protein it is bound to for transport to the bacterial periplasm, upon which it is cleaved off. This action can bolster the stability of some proteins, due to the relatively stronger reducing conditions prevalent in the bacterial periplasm.
This part is used in BBa_K1813052.

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Categories
Parameters
None