Part:BBa_K1796011

nifN promoted from Paenibacillus sp. WLY78

Function: scaffold gene of nitrogen fixation system

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 319
  Illegal PstI site found at 890
  Illegal PstI site found at 961
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 319
  Illegal PstI site found at 890
  Illegal PstI site found at 961
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 319
  Illegal PstI site found at 890
  Illegal PstI site found at 961
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 319
  Illegal PstI site found at 890
  Illegal PstI site found at 961
  Illegal NgoMIV site found at 648
  Illegal AgeI site found at 25
  Illegal AgeI site found at 147
  Illegal AgeI site found at 766
  Illegal AgeI site found at 987
  
• 1000
  COMPATIBLE WITH RFC[1000]

Parameter of Protein

Number of amino acids: 1434

Molecular weight: 118547.3

Theoretical pI: 4.97

Amino acid composition:聽

Ala (A) 316 22.0%

Arg (R) 0 0.0%

Asn (N) 0 0.0%

Asp (D) 0 0.0%

Cys (C) 365 25.5%

Gln (Q) 0 0.0%

Glu (E) 0 0.0%

Gly (G) 402 28.0%

His (H) 0 0.0%

Ile (I) 0 0.0%

Leu (L) 0 0.0%

Lys (K) 0 0.0%

Met (M) 0 0.0%

Phe (F) 0 0.0%

Pro (P) 0 0.0%

Ser (S) 0 0.0%

Thr (T) 351 24.5%

Trp (W) 0 0.0%

Tyr (Y) 0 0.0%

Val (V) 0 0.0%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 0

Total number of positively charged residues (Arg + Lys): 0

Atomic composition:Carbon C 4251

Hydrogen H 7070

Nitrogen N 1434

Oxygen O 1786

Sulfur S 365

Formula: C4251H7070N1434O1786S365Total number of atoms: 14906

Extinction coefficients:This protein does not contain any Trp residues. Experience shows that

this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 22750

Abs 0.1% (=1 g/l) 0.192, assuming all pairs of Cys residues form cystines

Ext. coefficient 0

Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is G (Gly).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 44.79

This classifies the protein as unstable.

Aliphatic index: 22.04

Grand average of hydropathicity (GRAVY): 0.750