Coding
HisTag-HIC

Part:BBa_K1602018

Designed by: Sebastian Barthel, Jonas Sindlinger, Bianca Reisinger, Sven Jager   Group: iGEM15_TU_Darmstadt   (2015-09-16)
Revision as of 01:41, 19 September 2015 by S Barthel (Talk | contribs)

Humicola insolens cutinase with N-terminal His-Tag

Humicola insolens cutinase with N-terminal His-Tag

cadA is a gene, coding for a cis-aconitate decarboxylase in Aspergillus terreus. The decarboxylase catalyzes the conversion from cis-aconitate to itaconic acid. The reaction takes place in the cytosol of the host and frees one molecule of carbon dioxide per conversion. 

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Characteristics


Molecular Weight 21068.85
Residues 200
Charge 5.5
Isoelectric Point 8.1624
A280 Molar Extinction Coefficients 12950 (reduced) 13200 (cystine bridges)
Improbability of expression in inclusion bodies 0.891
[Data taken from PEPSTATS]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None