Coding
HisTag-HIC

Part:BBa_K1602018

Designed by: Sebastian Barthel, Jonas Sindlinger, Bianca Reisinger, Sven Jager   Group: iGEM15_TU_Darmstadt   (2015-09-16)
Revision as of 01:40, 19 September 2015 by S Barthel (Talk | contribs)

Humicola insolens cutinase with N-terminal His-Tag

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Humicola insolens cutinase with N-terminal His-Tag

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Characteristics


               cadA is a gene, coding for a cis-aconitate decarboxylase in Aspergillus terreus. The decarboxylase catalyzes the conversion from cis-aconitate to itaconic acid. The reaction takes place in the cytosol of the host and frees one molecule of carbon dioxide per conversion.
Molecular Weight 21068.85
Residues 200
Charge 5.5
Isoelectric Point 8.1624
A280 Molar Extinction Coefficients 12950 (reduced) 13200 (cystine bridges)
Improbability of expression in inclusion bodies 0.891
[Data taken from PEPSTATS]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None